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Systematic alteration of the nucleotide sequence preceding the translation initiation codon and the effects on bacterial expression of the cloned SV40 small-t antigen gene

โœ Scribed by Gheysen, Dirk; Iserentant, Dirk; Derom, Catherine; Fiers, Walter


Book ID
121949383
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
741 KB
Volume
17
Category
Article
ISSN
0378-1119

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Expression cassettes containing a duplicated cauliflower mosaic virus (CaMV) 35S promoter fused to a polylinker preceded by the CCACCATGG and AACAATGG sequences were constructed. These two sequences correspond to the consensus sequences around the translation start codons in vertebrates and plants r