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Effect of two consensus sequences preceding the translation initiator codon on gene expression in plant protoplasts

✍ Scribed by F. Guerineau; A. Lucy; P. Mullineaux

Publisher: Springer
Year: 1992
Tongue: English
Weight: 285 KB
Volume: 18
Category: Article
ISSN: 0167-4412
DOI: 10.1007/bf00020027

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✦ Synopsis

Expression cassettes containing a duplicated cauliflower mosaic virus (CaMV) 35S promoter fused to a polylinker preceded by the CCACCATGG and AACAATGG sequences were constructed. These two sequences correspond to the consensus sequences around the translation start codons in vertebrates and plants respectively. Translational fusions were made with the fl-glucuronidase-coding sequence and transient expression was recorded in tobacco mesophyll protoplasts. Approximately three times more G U S activity was found in protoplasts incubated with the constructs harbouring translational fusions as compared to a control harbouring a transcriptional fusion. No significant difference was observed between G U S activities obtained with the two consensus sequences.

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