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Effect of deletions 5′ to the translation initiation sequence on the expression of an mRNA in animal cells

✍ Scribed by M. C. Ganoza; N. A. Farrow; G. An

Publisher
Springer
Year
1992
Tongue
English
Weight
543 KB
Volume
16
Category
Article
ISSN
0301-4851

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✦ Synopsis

To learn if an mRNA. 18S rRNA interaction or a special secondary structure in the mRNA start region is essential for translation in eukaryotic cells, we constructed recombinant plasmids with the SV40 early promoter 5' to part of the Escherichia coli tufB-lacZ gene. Deletion of bases potentially complementary to the 18S rRNA highly increased the transient fl-galactosidase expressed in transfected CHO cells. Deletion of bases that fostered formation of potential hairpins with the mRNA 5'-terminus or altered the structure of the coding region reduced fl-galactosidase activity suggesting that these features of the mRNA secondary structure may be essential for initiation of translation. Computer aided analysis of the potential structure of 290 mRNAs suggests these are conserved features of the initiation region.

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