Synthetic substrates for vertebrate collagenase
โ Scribed by Yoshihiro Masui; Tadashi Takemoto; Shumpei Sakakibara; Hisae Hori; Yutaka Nagai
- Book ID
- 115766601
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 339 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0006-2944
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๐ SIMILAR VOLUMES
Collagenase from normal human skin fibroblasts was found to catalyze the hydrolysis of esters and thio esters. This observation led to the development of a rapid, sensitive, continuous spectrophotometric assay for vertebrate collagenase using the thio peptolide Ac-ProLeuGly-S-LeuLeuGly-OCzHs as subs
## Abstract A synthetic collagenase substrate containing the internal peptide sequenceโGlyโGlyโProโLeuโGlyโProโProโGlyโProโhas been synthesized, with an Nโterminus 4โ((4โ(dimethylamino)phenyl)azo)โbenzoyl (DABCYL) group and Cโterminus 5โ[2โ(acetamido)ethylamino] naphthaleneโ1โsulfonic acid (AEDANS)