Synthetic Protein Switches: Methods and Protocols

✍ Scribed by Viktor Stein (eds.)

Publisher: Humana Press
Year: 2017
Tongue: English
Leaves: 332
Series: Methods in Molecular Biology 1596
Edition: 1
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

This detailed book provides state-of-the-art protocols to facilitate the construction of synthetic protein switches for a variety of applications in biotechnology and basic research. Beginning with general strategies, the volume continues with coverage of peptide switches, fluorescent and bioluminescent sensors, β-lactamase sensors, proteolytic sensors, optogenetic switches, as well as cellular signaling switches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Synthetic Protein Switches: Methods and Protocols serves as a useful reference for life scientists from diverse research fields that range from traditional, discovery-centered disciplines, like cancer research, to newly emerging disciplines, such as synthetic biology.

✦ Table of Contents

Front Matter....Pages i-xi
Front Matter....Pages 1-1
Front Matter....Pages 3-25
Front Matter....Pages 27-41
Front Matter....Pages 43-55
Front Matter....Pages 57-57
Front Matter....Pages 59-68
Front Matter....Pages 69-69
Back Matter....Pages 71-87
....Pages 89-99

✦ Subjects

Biotechnology

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