Cell-free Protein Synthesis: Methods and Protocols

✍ Scribed by Jennifer E. Van Eyk, Michael J. Dunn

Publisher: Wiley-VCH
Year: 2008
Tongue: English
Leaves: 696
Edition: 1
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latest advances in the field. The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike.

✦ Table of Contents

Cover......Page 1
Clinical Proteomics......Page 2
Related Titles......Page 3
Title......Page 4
Copyright......Page 5
Contents......Page 6
Editor’s Overview......Page 20
List of Contributors......Page 22
Part I: Technologies......Page 36
1 Preanalytical Issues in Clinical Proteomic Studies......Page 38
2 Protein Separation by Two-Dimensional Electrophoresis......Page 48
3 Protein Separation: Liquid Chromatography......Page 66
4 HPLC in Protein Discovery......Page 88
5 IEF Analysis of Peptides for Biomarkers Discovery......Page 96
6 Capillary Electrophoretic Separations for Clinical Proteomics......Page 108
7 Quantitative Proteomics Using Nano-LC with High Accuracy Mass Spectrometry......Page 124
8 Antibody Microarrays for Protein and Glycan Detection......Page 136
9 Biomarker Identification: The Role of Experimental Design, Statistics, and Data Sharing......Page 148
Part II: Cancer......Page 156
10 Applications of Stable Isotope Tagging Based Quantitative Proteomics in Cancer Research......Page 158
11 Two-Dimensional Liquid Separations, Protein Microarrays, and Mass Spectrometry in Comprehensive Analysis of Posttranslational Modifications and Biomarker Discovery in Cancers......Page 180
12 Development and Use of Reversed-Phase Protein Microarrays for Clinical Applications......Page 200
13 Cyclin-Dependent Kinase Inhibitors and Cancer: Usefulness of Proteomic Approaches in Assessment of the Molecular Mechanisms and Efficacy of Novel Therapeutics......Page 212
Part III: Cardiac Disease......Page 238
14 Diagnostic Markers for Monitoring Heart Transplant Rejection......Page 240
15 The Study of Microheterogeneity in Human Plasma Proteins: Application to Acute Myocardial Infarction......Page 252
16 Discovery of Biomarkers for Cardiovascular Diseases......Page 266
17 Development of Biomarker Development Pipeline: Search for Myocardial Ischemia Biomarkers......Page 276
18 The Albuminome as a Tool for Biomarker Discovery......Page 298
19 Application of Metabolomics to Cardiovascular Biomarker and Pathway Discovery......Page 314
Part IV: Vascular Disease: Pulmonary, Diabetes and Brain......Page 330
20 Urinary Biomarkers in Diabetic Nephropathy and Other Glomerular Diseases......Page 332
21 Pulmonary Proteomics......Page 358
22 Proteomics Providing Insights into Major Psychiatric Disorders......Page 384
Part V: Toxicity, Bacterial and Viral Infections......Page 414
23 Redox Proteomics Analysis of Oxidative Modified Brain Proteins in Alzheimer's Disease and Mild Cognitive Impairment: Insights into the Progression of This Dementing Disorder......Page 416
24 Toxicoproteomics: Correlating Tissue and Serum Proteomics in Liver Injury......Page 438
25 Biomarkers for Renal Disease and Uremic Toxins......Page 470
26 HIV and Other Viral Screens......Page 488
Part VI: Autoimmune Disease and Autoantibodies......Page 516
27 Application of Fungal Cyclic Peptides and Metabolites......Page 518
28 Microarray Approaches to Autoantibody Profiling......Page 546
29 Identification of Tumor Antigen Directed Autoantibodies......Page 568
30 Antibody and Reverse Capture Protein Microarrays for Clinical Proteomics......Page 584
31 Use of Antibody Microarrays in the Analysis of Inflammation, Autoimmunity, Viral Infection, and Cancer Metastases......Page 606
Part VII: Translation: Discovery to the Clinic......Page 628
32 The Future: Translation from Discovery to the Clinic – Roles of HUPO and Industry in Biomarker Discovery......Page 630
33 Requirements of a Good Biomarker: Translation into the Clinical Laboratory......Page 650
34 Translation of Protein Biomarkers for Clinical Development......Page 668
Index......Page 688
Back Cover......Page 696

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