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Synthetic peptide analogs to probe the immunological expression of the rat encephalitogenic neuropeptide

✍ Scribed by G. A. Hashim; E. D. Day

Book ID
102385868
Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
456 KB
Volume
18
Category
Article
ISSN
0360-4012

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✦ Synopsis

The rationale for designing and synthesizing 24 different peptide analogs and subpeptides of residues 69-84 of guinea pig myelin basic protein is presented. The encephalitogenic potential of 19 of these peptides in Lewis rats is also given.

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Immunochemical analysis of polyclonal antisera from Lewis rats immunized with peptide analogs and subpeptides of residues 72-84 of guinea pig myelin basic protein (MBP) indicated that there were four main epitopic specificities involved: one represented by residues 76-80, one by residues 72-74, one

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The antibody recognition of synthetic rabbit encephalitogen S24 (TTHYGSLPQKG), which had been inactivated by extensive iodination, was fully restored by tritium-iodine exchange during boron hydride reduction. A highly active form of 3H-S24 was obtained by reverse-phase high-performance liquid chroma