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Synthesis, purification and bioactivity of recombinant human activin A expressed in the yeast Pichia pastoris

โœ Scribed by Theo Papakonstantinou; Simon J. Harris; Dale Fredericks; Craig Harrison; Euan M. Wallace; Milton T.W. Hearn

Book ID
116828921
Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
786 KB
Volume
64
Category
Article
ISSN
1046-5928

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โœ Dale Fredericks; Robert Clay; Tracy Warner; Anne O'Connor; David M. de Kretser; ๐Ÿ“‚ Article ๐Ÿ“… 2010 ๐Ÿ› American Institute of Chemical Engineers ๐ŸŒ English โš– 841 KB

## Abstract We report a new procedure to express recombinant human activin A using the methanolic yeast, __Pichia pastoris__. Optimization of culture procedures has involved comprehensive examination of the effects of culture vessel shape, volume of broth in the induction and expression cultures, m