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Synthesis of two carboxylic haptens for raising antibodies to 25-hydroxyvitamin D3 and 1α,25-dihydroxyvitamin D3

✍ Scribed by Carlos Gregorio; Silvina Eduardo; Luis C. Rodrigues; María A. Regueira; Ramón Fraga; Ricardo Riveiros; Miguel Maestro; Antonio Mouriño

Book ID
116698004
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
393 KB
Volume
103
Category
Article
ISSN
0960-0760

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✍ Joji Sasaki; Akiko Miyazaki; Mika Saito; Takashi Adachi; Kazutoshi Mizoue; Kazun 📂 Article 📅 1992 🏛 Springer 🌐 English ⚖ 580 KB

To enzymatically synthesize active metabolites of vitamin D3, we screened about 500 bacterial strains and 450 fungal strains, of which 12 strains were able to convert vitamin D 3 to lc~,25-dihydroxyvitamin D3 [lc~,25(OH)2.D3] via 25-hyroxyvitamin D3 [25(OH)Da]. The conversion activity was only detec