Synthesis of the bradykinin B1antagonist [desArg10]HOE 140 on 2-chlorotrityl resin

✍ Scribed by Paolo Rovero; Stefania Viganò; Stefano Pegoraro; Laura Quartara

• Book ID: 104628866
• Publisher: Springer Netherlands
• Year: 1996
• Tongue: English
• Weight: 327 KB
• Volume: 2
• Category: Article
• ISSN: 1573-3149
• DOI: 10.1007/bf00119994

✦ Synopsis

This paper describes the synthesis of the bradykinin B l antagonist [desArgl°]HOE 140 (D-Arg-Arg-Pro-Hyp-Gly-Thi-Ser-D-Tic-Oic-OH) by the solid-phase method. This synthesis is predicted to be a difficult one because the Cterminal sequence D-Tic-Oic, when linked to the resin, could easily undergo an intramolecular aminolysis, releasing the corresponding diketopiperazine. This reaction is greatly favored by the imino acidic structure of these two residues and by the D-configuration of the second one. When using the Fmoc strategy, the reaction takes place during the Fmoc removal with piperidine. In this case, it has been suggested previously that it is possible to prevent the side reaction by reducing the time of the base treatment. In our hands, this expedient worked correctly for the synthesis of a test tetrapeptide (H-GIy-Pro3-OH), but under the same conditions the synthesis of the target peptide failed completely. In contrast, the use of the very hindered 2-chlorotrityl resin reduced diketopiperazine formation to undetectable levels.