Synthesis of Tc-99m-labeled, modified RNA

✍ Scribed by Christoph S. Hilger; Michael C. Willis; Mark Wolters; Wolfgang A. Pieken

• Book ID: 104260322
• Publisher: Elsevier Science
• Year: 1998
• Tongue: French
• Weight: 166 KB
• Volume: 39
• Category: Article
• ISSN: 0040-4039
• DOI: 10.1016/s0040-4039(98)02193-5

✦ Synopsis

The synthesis of Tc-99m-labeled, modified RNA is reported. This new class of radiopharmaceuticals is of potential interest as target specific imaging agents. The preparation of NaS-conjugated RNA was achieved by coupling S-protected MAG2-units to amino modified RNA in solution or on solid support. The starting S-protected MAG2 building blocks (1R-S-CH2-CO-Gly-Gly-2R: 1R = Ac, tBu-S; 2R = OH, OSu) were obtained by a simple 4-or 5-step synthesis. The MAG2amide-RNA-conjugates were successfully Tc-99m-labeled with high yield and specific activities of 37MBq/nmol leading to 1:1-Tc-99m-N3S-aptamers.