Synthesis of [Sar1, Val5, (4′-Azido-3′,5′-ditritio)Phe8] Angiotensin II, a Photoaffinity Label for the Isolation of Angiotensin II Receptors Communication 1

✍ Scribed by Michel Bernier; Emannuel Escher

• Publisher: John Wiley and Sons
• Year: 1980
• Tongue: German
• Weight: 219 KB
• Volume: 63
• Category: Article
• ISSN: 0018-019X
• DOI: 10.1002/hlca.19800630523

✦ Synopsis

Abstract

The synthesis of [Sar^1^, Val^5^, (4′‐azido‐3′,5′‐ditritio)Phe^8^] angiotensin II from a iodinated precursor peptide is described. The principal problems of this synthesis and their resolution are discussed: (i) The β‐induced autophotolysis of the highly tritiated (73 Ci/mmol) and photosensitive label, and (ii) the absorption problems encountered during the purification of microgramm quantities. Such photoaffinity labels are being used for specific labeling and isolation of peptide hormone receptors.