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Synthesis of rhodanese in Hep 3B cells

✍ Scribed by Rita Pallini; Carlo Cannella; Paola Natale

Publisher
Springer
Year
1990
Tongue
English
Weight
477 KB
Volume
93
Category
Article
ISSN
0300-8177

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✦ Synopsis

The biosynthesis of rhodanese was studied in human hepatoma cell lines by immunoblotting and pulse-labeling experiments using polyclonal antibodies raised against the bovine liver enzyme. Rhodanese, partially purified from human liver, showed an apparent molecular weight of 33,000 daltons, coincident with that of rhodanese from Hep 3B cells. After pulse labeling of Hep 3B cells both at 37 degrees C and 25 degrees C, rhodanese in the cytosol fraction exhibited the same molecular weight as the enzyme isolated from the particulate fraction containing mitochondria. Moreover, newly synthesized rhodanese from total Hep 3B RNA translation products showed the same electrophoretic mobility as rhodanese from Hep 3B cells. These results suggest that rhodanese, unlike most mitochondrial proteins, is not synthesized as a higher molecular weight precursor.

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