The preparation of N(2)-phenoxylacetyl-S(6)-(2,4-dinitrophenyl)-6-thioguanosine phosphoramidite and its subsequent incorporation into oligoribonucleotides is described. The identity of the oligonucleotides was confirmed by UV spectrophotometry and nucleoside composition analysis.
Synthesis of pyridinone ribonucleoside 3′-O-phosphoramidites and their incorporation into oligoribonucleotides
✍ Scribed by Jasenka Matulic-Adamic; Carolyn Gonzalez; Nassim Usman; Leonid Beigelman
- Book ID
- 108038974
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 319 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0960-894X
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
## Abstract We present optimized reaction conditions for the conversion of 2′‐__O__‐{[(triisopropylsilyl)oxy]methyl}(=tom) protected uridine and adenosine nucleosides into the corresponding protected (3‐^15^N)‐labeled uridine and cytidine and (1‐^15^N)‐labeled adenosine and guanosine nucleosides **
Oligoribonucleotides, six to fifteen units long, were synthesized on a controlled pore glass support using ribonucleoside 3'-0-phosphoramidite reagents. Average coupling yieids of up to 98% were obtained with dirsopropylamrnophosphoramidite derivatives. In light of the increasing interest in the ro