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Synthesis of polygalacturonase during tomato fruit ripening

โœ Scribed by Gregory A. Tucker; Donald Grierson

Publisher
Springer-Verlag
Year
1982
Tongue
English
Weight
580 KB
Volume
155
Category
Article
ISSN
0032-0935

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โœฆ Synopsis

The cell wall degrading enzyme polygalacturonase (E.C. 3.2.1.15) is not detectable in green tomatoes (Lycopersicon esculentum Mill). Activity appears at the onset of ripening and in ripe fruit it is one of the major cell-wall-bound proteins. Radioimmunoassay results, employing an antibody against purified polygalacturonase, suggest that during ripening the enzyme is synthesised de novo. Radioimmunoassay data also show that the low level of polygalacturonase in "Never ripe" mutants and the lack of activity in "ripening inhibitor" mutants can be correlated to the levels of immunologically detectable polygalacturonase protein.

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โœ Russell Pressey ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 496 KB

A procedure was developed for the differential extraction of polygalacturonases (PG) I and II from tomatoes (Lycopersicon esculentum Mill.). Extraction of pericarp tissue from ripe fruit at conventional conditions of 1.0 M NaC1 and pH 6.0 yielded nearly equal amounts of the two enzymes. However, mos