The cell wall degrading enzyme polygalacturonase (E.C. 3.2.1.15) is not detectable in green tomatoes (Lycopersicon esculentum Mill). Activity appears at the onset of ripening and in ripe fruit it is one of the major cell-wall-bound proteins. Radioimmunoassay results, employing an antibody against pu
Molecular characterization of tomato fruit polygalacturonase
โ Scribed by Sheehy, Raymond E. ;Pearson, Judith ;Brady, Colin J. ;Hiatt, William R.
- Publisher
- Springer
- Year
- 1987
- Tongue
- English
- Weight
- 729 KB
- Volume
- 208
- Category
- Article
- ISSN
- 0026-8925
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Autolysis of cell walls from polygalacturonase (PG)-antisense tomato fruit was studied in a conventional buffer designed to maximize the catalytic activity of PG (30 mM sodium acetate, 150 mM NaCl, pH 4.5), and in solutions mimicking the pH and mineral composition of the fruit apoplast at the mature
Differential hybridization was used to screen a cDNA library made from ripe tomato fruit poly(A +)RNA. Clones were identified representing genes expressed predominantly at the unripe and/or ripe stage of the fruit development. Northern analysis was used for further characterization of the clones and