Synthesis of P1,P2-dinucleotide pyrophosphates
β Scribed by R van der Woerd; C.G Bakker; Alan W Schwartz
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- French
- Weight
- 185 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0040-4039
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## Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a βFull Textβ option. The original article is trackable v
A novel s y n t h e t i c approach has been developed f o r t h e s y n t h e s i s of i n o r g a n i c pyrophosphate which a l l o w s t h e i s o t o p e s 170 and " 0 t o be i n c o r p o r a t e d a t P' t o g i v e c h i r a l l y l a b e l l e d material.
A simple procedure for synthesizing [32P]pyrophosphate with high specific activity (about 100 Ci/mmol) is described. [32P]Pyrophosphate was formed by simple dehydration of inorganic [3\*P]phosphate and subsequently purified by DEAE-Sephadex A-25 chromatography. The yield was 20-40%.