Synthesis of Oligoribonucleotides Containing 2-Thiouridine: Incorporation of 2-Thiouridine Phosphoramidite without Base Protection
β Scribed by Kumar, Raju K.; Davis, Darrell R.
- Book ID
- 127370784
- Publisher
- American Chemical Society
- Year
- 1995
- Tongue
- English
- Weight
- 281 KB
- Volume
- 60
- Category
- Article
- ISSN
- 0022-3263
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π SIMILAR VOLUMES
A r&able prqxwation of S%yanm~y~4-th ad its incorporation into oligoribnucleoddes is reported. Deproteciion qf oligo&otuuleotidks with DBU in acetonitrile followed by methanolic amraonio allmvs the use of standard N-benwyl and N-isoburyrylp7otectedp~~~r~t~. Cleavage of hammerhead rikqvmes using GCG
The syntheses of protected derivatives of 5-methylaminomethyl-2-thiouridine(mnm5s2U)11 \_, IJ and 5-carbomethoxymethyl-2-thiouridine(mcm5s2U)13 as well as their unprotected 3'-phosphates 3, 15 have been described.
The preparation of N(2)-phenoxylacetyl-S(6)-(2,4-dinitrophenyl)-6-thioguanosine phosphoramidite and its subsequent incorporation into oligoribonucleotides is described. The identity of the oligonucleotides was confirmed by UV spectrophotometry and nucleoside composition analysis.