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Synthesis of Nucleoside Triphosphates that Contain an Aminooxy Function for “Post-Amplification Labelling”

✍ Scribed by Emmanuelle Trévisiol; Eric Defrancq; Jean Lhomme; Ali Laayoun; Philippe Cros

Book ID: 101274460
Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 333 KB
Volume: 2000
Category: Article
ISSN: 1434-193X
DOI: 10.1002/(sici)1099-0690(200001)2000:1<211::aid-ejoc211>3.0.co;2-v

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✦ Synopsis

Preparation of the uridine and adenosine triphosphates 1 and fluorescein derivative 15. They could each be incorporated into a 330-mer fragment with T7 RNA polymerase. 2 bearing a linker with a terminal aminooxy group is described. Both 1 and 2 react readily with the aldehydic ized amplicons should be stable for the subsequent readout.

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