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Synthesis of Methylketone Containing Nucleoside Triphosphates for RNA Labelling

✍ Scribed by Emmanuelle Trévisiol; Eric Defrancq; Jean Lhomme; Ali Laayoun; Philippe Cros

Book ID
104202880
Publisher
Elsevier Science
Year
2000
Tongue
French
Weight
203 KB
Volume
56
Category
Article
ISSN
0040-4020

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✦ Synopsis

AbstractÐThe three nucleoside triphosphates 1, 2 and 3 bearing a linker with a terminal methylketone group were prepared for incorporation into RNA fragments and post-labelling by the ¯uorescein derivative 4 containing an aminooxy group.

📜 SIMILAR VOLUMES

Synthesis of Nucleoside Triphosphates th
Synthesis of Nucleoside Triphosphates that Contain an Aminooxy Function for “Post-Amplification Labelling”
✍ Emmanuelle Trévisiol; Eric Defrancq; Jean Lhomme; Ali Laayoun; Philippe Cros 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 333 KB

Preparation of the uridine and adenosine triphosphates 1 and fluorescein derivative 15. They could each be incorporated into a 330-mer fragment with T7 RNA polymerase. 2 bearing a linker with a terminal aminooxy group is described. Both 1 and 2 react readily with the aldehydic ized amplicons should