Synthesis of nucleic acids and localization of atrial natriuretic peptide in crayfish haemocytes
β Scribed by M. G. Martynova; O. A. Bystrova; V. N. Parfenov
- Book ID
- 111471454
- Publisher
- Pleiades Publishing
- Year
- 2008
- Tongue
- English
- Weight
- 695 KB
- Volume
- 2
- Category
- Article
- ISSN
- 1990-519X
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Peptide nucleic acids (PNA) were synthesized by a modified Merrifield method using several improvements. Activation by O-[benzotriazol-1-yl]-1,1,3,3-tetramethyluronium hexafluorophosphate in combination with in situ neutralization of the resin allowed efficient coupling of all four Boc-protected PNA
Atrial natriuretic peptide (ANP) is a clinically useful anti-hypertensive hormone. Maleimide derivatives of ANP have been synthesized and conjugated to cysteine-34 of human serum albumin. The conjugates were analyzed to assess their stability, receptor binding affinity and ability to stimulate guany