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Synthesis of multiply labelled ribonucleosides for sequence-specific labelling of oligo-RNA

✍ Scribed by Jan Milecki; Andras Földesi; Artur Fischer; Ryszard W. Adamiak; Jyoti Chattopadhyaya

Book ID
102371374
Publisher
John Wiley and Sons
Year
2001
Tongue
French
Weight
221 KB
Volume
44
Category
Article
ISSN
0022-2135

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✦ Synopsis

Abstract

The synthesis of ribonucleotide blocks multiply labelled with ^2^H, ^13^C and ^15^N for solid support synthesis of sequence specifically labelled RNA is described. Labels were introduced in the ribose ring (^13^C), C5 position of pyrimidine nucleobases (^2^H) and exocyclic amino groups (^15^N) and serve as multiple probes for studying the various physicochemical consequences of physiologically important RNA folding by high‐resolution multi‐nuclear NMR spectroscopy. Copyright © 2001 John Wiley & Sons, Ltd.

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