✦ LIBER ✦

Synthesis of immunoglobulin and nuclear protein in synchronized mouse myeloma cells

✍ Scribed by P. Liberti; C. Baglioni

Publisher: John Wiley and Sons
Year: 1973
Tongue: English
Weight: 586 KB
Volume: 82
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040820113

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✦ Synopsis

Abstract

The synthesis of immunoglobulin and of nuclear proteins has been studied in synchronized mouse myeloma cells of the C1 line. Synchronization has been obtained by a double thymidine block. C1 cells synthesize immunoglobulin at a relatively constant rate throughout the cell cycle except for mitosis, when a decrease in the rate of synthesis of total protein and of immunoglobulin is observed. Cell synchrony around mitosis is not sufficiently good to determine whether immunoglobulin is synthesized at all. Nuclear protein and in particular histones appear to be synthesized synchronously with DNA during the S phase of the cell cycle.

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