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Synthesis of heptapeptides and analysis of sequence by tandem ion trap mass spectrometry

✍ Scribed by Jia, Chenxi ;Qi, Wei ;He, Zhimin ;Yang, Haoming ;Qiao, Bin

Book ID
111487886
Publisher
Walter de Gruyter GmbH
Year
2006
Tongue
English
Weight
306 KB
Volume
4
Category
Article
ISSN
2391-5420

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✦ Synopsis

Abstract

Two heptapeptides have been prepared by Fmoc methodology using Wang resin as solid support. For attachment of the first amino acid, several coupling systems were evaluated, and DIC/DMAP system could give yields of >99% and low levels of racemization. The selection of scavenger combination to deprotect side chains revealed that H2O/p-cresol was good at scavenging trityl and 1,2-ethanedithiol was highly efficient for scavenging t-butyl. Through shortening the preactivation time to 5 min, the racemization which occurred during formation of amide bonds coupled by HBTU was minimized. The crude peptides were characterized by RP-HPLC and MS, and sequenced by MS/MS to acquire reliable amino acid sequence information.

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