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Synthesis of gefarnate with reusable lipase modified with polyethylene glycol

โœ Scribed by Mizutani, Akemi; Takahashi, Katsunobu; Aoki, Takashi; Ohwada, Kimiko; Kondo, Kiyosi; Inada, Yuji


Book ID
121895335
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
236 KB
Volume
10
Category
Article
ISSN
0168-1656

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Monomethoxypolyethylene glycols (PEG) of molecular masses 1900 and 5000 were activated using p-nitrophenyl chloroformate to form PEGnitrophenyl carbonates (activated PEG) with high yield (96-98%). The activated PEG was covalently attached to Candida rugosa lipase. Increasing the molar ratio of activ