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Ester-exchange catalyzed by lipase modified with polyethylene glycol

โœ Scribed by K. Takahashi; Y. Kodera; T. Yoshimoto; A. Ajima; A. Matsushima; Y. Inada


Book ID
115758840
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
288 KB
Volume
131
Category
Article
ISSN
0006-291X

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Monomethoxypolyethylene glycols (PEG) of molecular masses 1900 and 5000 were activated using p-nitrophenyl chloroformate to form PEGnitrophenyl carbonates (activated PEG) with high yield (96-98%). The activated PEG was covalently attached to Candida rugosa lipase. Increasing the molar ratio of activ