Retinyl esters synthesis by polyethylene glycol-modified lipase in benzene
โ Scribed by Ayako Ajima; Katsunobu Takahashi; Ayako Matsushima; Yuji Saito; Yuji Inada
- Publisher
- Springer Netherlands
- Year
- 1986
- Tongue
- English
- Weight
- 265 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0141-5492
No coin nor oath required. For personal study only.
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Monomethoxypolyethylene glycols (PEG) of molecular masses 1900 and 5000 were activated using p-nitrophenyl chloroformate to form PEGnitrophenyl carbonates (activated PEG) with high yield (96-98%). The activated PEG was covalently attached to Candida rugosa lipase. Increasing the molar ratio of activ
Chymotrypsin modified with polyethylene glycol was successfully used for peptide synthesis in organic solvents. The benzene-soluble modified enzyme readily catalyzed both aminolysis of N-benzoyl-L-tyrosine p-nitroanilide and synthesis of N-benzoyl-L-tyrosine butylamide in the presence of trace amoun