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Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides

✍ Scribed by Gibson, D. G.

Book ID
127180522
Publisher
Oxford University Press
Year
2009
Tongue
English
Weight
348 KB
Volume
37
Category
Article
ISSN
0305-1048

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✍ Gibson, Daniel G. πŸ“‚ Article πŸ“… 2011 πŸ› Elsevier 🌐 English βš– 306 KB

Three methods for assembling multiple, overlapping DNA molecules are described. Each method shares the same basic approach: (i) an exonuclease removes nucleotides from the ends of double-stranded (ds) DNA molecules, exposing complementary single-stranded (ss) DNA overhangs that are specifically anne