[Methods in Enzymology] Synthetic Biolog
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Gibson, Daniel G.
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Article
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2011
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Elsevier
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English
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Three methods for assembling multiple, overlapping DNA molecules are described. Each method shares the same basic approach: (i) an exonuclease removes nucleotides from the ends of double-stranded (ds) DNA molecules, exposing complementary single-stranded (ss) DNA overhangs that are specifically anne