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Synthesis of carbamoyl phosphate by carbamoyl phosphate synthetase I in the absence of acetylglutamate. Activation of the enzyme by cryoprotectants

✍ Scribed by Hubert Greenslade Britton; Vicente Rubio; Santiago Grisolia


Book ID
118315394
Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
446 KB
Volume
99
Category
Article
ISSN
0006-291X

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An indirect, competitive enzyme-linked immunosorbent assay for the quantitation of carbamoyl-phosphate synthetase I (ammonia) in rat liver has been developed. Homogenization of the liver in 1% sodium deoxycholate is used for complete solubilization of the enzyme. The detergent does not interfere wit