✦ LIBER ✦

An enzyme immunoassay for the quantitation of rat liver carbamoyl-phosphate synthetase I

✍ Scribed by M. Cerda; A. Jorda; T. Barber; J.V. Castell; J. Cabo; J. Timoneda

Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 613 KB
Volume: 174
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(88)90074-7

No coin nor oath required. For personal study only.

✦ Synopsis

An indirect, competitive enzyme-linked immunosorbent assay for the quantitation of carbamoyl-phosphate synthetase I (ammonia) in rat liver has been developed. Homogenization of the liver in 1% sodium deoxycholate is used for complete solubilization of the enzyme. The detergent does not interfere with the method if diluted to a concentration of 0.01% or lower. The assay is applied to determine the amount of enzyme in control rats and in rats fed "cafeteria" or high-protein diets. Changes in the amount of carbamoyl-phosphate synthetase I (ammonia) paralleled changes in enzymatic activity.

📜 SIMILAR VOLUMES

An enzyme immunoassay for the quantitati

An enzyme immunoassay for the quantitation of dihydropteridine reductase

✍ Sau-Wah Kwan; Rong-sen Shen; Creed W. Abell 📂 Article 📅 1987 🏛 Elsevier Science 🌐 English ⚖ 1011 KB

Carbamoyl-phosphate synthase (ammonia) o

Carbamoyl-phosphate synthase (ammonia) of rat and axolotl liver: Determination of immunological cross-reactivity without purification of the axolotl enzyme

✍ Lamers, Wouter H. ;De Graaf, Andre ;Mooren, Piet G. ;Moorman, Antoon F. M. ;Char 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 English ⚖ 329 KB

## Abstract A method has been developed to establish the degree of cross‐reactivity of an antiserum raised against purified carbamoyl‐phosphate synthase (ammonia) from adult rat liver, toward a homologous enzyme from another species without purification of the latter enzyme. For that purpose the ra

An intronic silencer element is responsi

An intronic silencer element is responsible for specific zonal expression of glutamine synthetase in the rat liver

✍ Frank Gaunitz; Danilo Deichsel; Kerstin Heise; Max Werth; Ulf Anderegg; Rolf Geb 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 233 KB

The most striking phenomenon of glutamine synthetase (GS) expression in the liver is its unique restriction to cells surrounding the terminal hepatic venules. Expression is positively regulated by elements located in the 5'-upstream region and in the first intron of the gene. It was long believed th

An Enzyme Immunoreceptor Assay for the Q

An Enzyme Immunoreceptor Assay for the Quantitation of Insulin-like Growth Factor-I and Insulin Receptors in Bovine Muscle Tissue

✍ A. Boge; H. Sauerwein; H.H.D. Meyer 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 602 KB

New possibilities of enzymic synthesis o

New possibilities of enzymic synthesis of radioactive nucleotides. IV. Application of in vivo tryptophan-stabilized enzyme system from regenerating rat liver for the synthesis of radioactive thymidine 5′ - phosphates in vitro

✍ Jiri Filip; Karel Hybs; Zdenek Nejedly; Januaz Greger; Jiri Vesely; Alois Cihak 📂 Article 📅 1972 🏛 John Wiley and Sons 🌐 French ⚖ 313 KB

The e n z y m a t i c e x t r a c t p t e p a r e d f r o m 48h. r e g e n e r at i n g r a t l i v e r s t a b i l i z e d w i t h d i e t a r y L -t r y p t o p h a n p o s s e s s e s t h e enhanced t h y m i d i n e and t h y m i d y l a t e k inaae a c t i v i t y . The o p t i m a l c o n d i