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Synthesis of Branched Oligonucleotides as Templates for the Assembly of Nanomaterials

✍ Scribed by Marta G. Grimau; Daniela Iacopino; Anna Aviñó; Beatriz G. de la Torre; Andrea Ongaro; Donald Fitzmaurice; Jurina Wessels; Ramon Eritja

Publisher
John Wiley and Sons
Year
2003
Tongue
German
Weight
212 KB
Volume
86
Category
Article
ISSN
0018-019X

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✦ Synopsis

Abstract

Branched oligonucleotides with symmetric arms 6–15, which may contain biotin as a second recognition code, were prepared. These molecules are designed to be used for the directed assembly of nanomaterials. The branched structure of the desired oligonucleotides was confirmed by mass spectrometry on small branched oliognucleotides, by gel electrophoresis, and by hybridization with complementary oligonucleotidenanoparticle conjugates, followed by visualization of the complexes by transmission electron microscopy.

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