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Synthesis of Bacteriophage Lambda DNA in vitro: Requirement for O and P Gene Products

✍ Scribed by Hiroaki Shizuya and Charles C. Richardson

Book ID
123652383
Publisher
National Academy of Sciences
Year
1974
Tongue
English
Weight
843 KB
Volume
71
Category
Article
ISSN
0027-8424

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✍ Klinkert, JΓΆrg ;Klein, Albrecht πŸ“‚ Article πŸ“… 1979 πŸ› Springer 🌐 English βš– 844 KB

A restriction fragment of lambdaDNA carrying the P gene was cloned in the high copy number plasmid RSF2124. Cells harbouring this new plasmid RSF2124/lambdaE complement lambdaPam80 phage. A lac promoter-operator region (lacP), produced by EcoRI digestion of plasmid pKB252, was inserted into RSF2124/