Lysogenization by bacteriophage lambda IV inhibition of phage DNA synthesis by the products of genes cII and cIII
β Scribed by Kourilsky, Philippe; Gros, Danielle
- Book ID
- 122338617
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 503 KB
- Volume
- 58
- Category
- Article
- ISSN
- 0300-9084
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The activation of the int gene by the cII and cIII gene products was studied by analysing int expression following infection of UV-irradiated cells by various phage mutants. Residual expression of int, probably from Pl, takes place in the absence of cII/cIII activation. Activation of the int gene, l
A restriction fragment of lambdaDNA carrying the P gene was cloned in the high copy number plasmid RSF2124. Cells harbouring this new plasmid RSF2124/lambdaE complement lambdaPam80 phage. A lac promoter-operator region (lacP), produced by EcoRI digestion of plasmid pKB252, was inserted into RSF2124/