Synthesis of aspartame precursor: α-L-aspartyl-L-phenylalanine methyl ester in ethyl acetate using thermolysin entrapped in polyurethane

High isolated yields in thermolysin-cata

High isolated yields in thermolysin-catalysed synthesis of Z-l-aspartyl-l-phenylalanine methyl ester in toluene at controlled water activity

✍ Luigi De Martin; Cynthia Ebert; Lucia Gardossi; Paolo Linda 📂 Article 📅 2001 🏛 Elsevier Science 🌐 French ⚖ 57 KB

Aspartyl-L-phenylalanine methyl and ethyl esters were synthesised enzymatically in toluene by means of the zinc protease thermolysin adsorbed onto Celite R-640 ® , which is a porous support able to control the hydration of the protein. The conversion of the two derivatised amino acids into the desir