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Synthesis of aspartame precursor with an immobilized thermolysin in mixed organic solvents

โœ Scribed by Masamitsu Miyanaga; Takaaki Tanaka; Takaharu Sakiyama; Kazuhiro Nakanishi

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
455 KB
Volume
46
Category
Article
ISSN
0006-3592

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โœฆ Synopsis

N-(benzyloxycarbony1)-L-aspartyl-L-phenylalanine methyl ester, a precursor of the synthetic sweetener, aspartame, was synthesized from N-(benzyloxycarbony1)-L-as- partic acid and L-phenylalanine methyl ester with an immobilized thermolysin (EC 3.4.24.4) in the mixed organic solvent system of tert-amyl alcohol and ethyl acetate. A mixed solvent consisting of tert-amyl alcohol and ethyl acetate at a ratio of 33:67 (v/v) was found to be the most suitable with respect to synthetic rate and stability of the immobilized enzyme. The reaction continued to proceed quite successfully in a column reactor at 40ยฐC and at a space velocity of 3.6 h -' with a yield of 99%, using 40 m M Z-Asp and 200 m M PheOMe dissolved in the mixed solvent as the substrate.

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