Synthesis of allyl β-d-galactopyranoside from lactose using Streptococcus thermophilus β-d-galactosidase
✍ Scribed by David E. Stevenson; Richard H. Furneaux
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 242 KB
- Volume
- 284
- Category
- Article
- ISSN
- 0008-6215
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The enzyme-catalyzed hydration2 of D-galactal and the enzyme-catalyzed hydrolysis of fl-D-galactopyranosides are similar as far as the products are concerned. In the case of hydration of D-galactal, a covalent, 2deoxy-D-Zyxo-hexosy1("2deoxy-D-galactosyl")enzyme intermediate could be isolated' . As s
Disaccharide-peptide conjugates were obtained in yields of 30-50% from o-nitrophenyl beta-D-galactopyranoside by employing beta-D-galactosidase from E. coli as catalyst. Two series of beta-D-galactosyldipeptides were examined as galactosyl acceptors. They both contain an L-serine residue beta-linked