Synthesis of 6-amino-2-vinylpurine derivatives for cross-linking and evaluation of the reactivity
β Scribed by Kusano, Shuhei; Sakuraba, Tomoya; Hagihara, Shinya; Nagatsugi, Fumi
- Book ID
- 122011315
- Publisher
- Elsevier Science
- Year
- 2012
- Tongue
- English
- Weight
- 567 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0960-894X
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We previously reported that oligodeoxynucleotides containing 2-amino-6-vinylpurine (2-AVP: 1) exhibit efficient selective cross-linking to cytosine. In this study, the 2 0 -OMe nucleoside analogue (2) of 2-AVP was designed in order to increase its affinity to RNA and enhance metabolic stability. It
The 6-vinylated 2-aminopurine nucleoside (1), which was prepared by the Pd(0)-catalyzed cross-coupling reaction using guanosine 6-O-tosylate and vinyltributylstannane, has been demonstrated as a potential cross-linking agent. However, attempts for its incorporation into oligonucleotides were unsucce