Synthesis of 3?-O-(?-aminoalkoxymethyl)thymidine 5?-triphosphates, terminators of DNA synthesis that enable 3?-labelling
✍ Scribed by Hovinen, Jari; Azhayeva, Elena; Azhayev, Alex; Guzaev, Andrei; L�nberg, Harri
- Book ID
- 120060139
- Publisher
- Royal Society of Chemistry
- Year
- 1994
- Weight
- 992 KB
- Volume
- 2
- Category
- Article
- ISSN
- 1472-7781
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## Abstract An enzymatic method for synthesis of tritium‐labelled thymidine‐5′‐triphosphate is described. The procedure utilizes a nucleoside phosphotransferase from wheat germ and a partially purified thymidylate kinase from calf thymus. The product is isolated by ion‐exchange chromatography on DE