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Synthesis of 1-O-[hexadecyl-1′,2′-3H]hexadecyl 2-acetyl-sn-glyceryl 3-phosphorylcholine and 1-O-alkyl [32P]lysophosphatidycholine

✍ Scribed by Un Hoi Do; Yang Hong; Peter Tam; Puliyur Srinivasan

Publisher
John Wiley and Sons
Year
1996
Tongue
French
Weight
480 KB
Volume
38
Category
Article
ISSN
0022-2135

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✦ Synopsis

1 -0-[hexadecyl-1',2'-3H]Hexadecyl 2-acetyl-sn-glyceryl3-phosphorylcholine (GPC) was prepared by acetylation of 1-0-alkenyl lysophosphatidylcholine, reduction of the alkenyl lipid with tritium gas over palladium oxide, and separation of molecular species of 1-0-[3H]alkyl2-acetyl GPC by reverse phase thin-layer chromatography. I-O-Alkyl[32P]lysophosphatidylcholine was prepared by mild alkaline hydrolysis of I-0-alkyl 2-acyl [32P]phos hatidylcholine, which was 1-0-hexadecyl2-acetyl-sn-glycerol in the presence of rat liver microsomes. The resulting radiolabeled ether phospholipids possessed high specific radioactivity suitable for metabolic and binding studies. enzymatically produced by treating cytidine 5'-[ 3 l P]diphosphocholine with

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