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Synthesis, Characterization and Properties of ppp(A2′p)nApCp and Related High-Specific-Activity 32P-Labelled Derivatives of ppp(A2′p)nA

✍ Scribed by Robert H. SILVERMAN; Christopher S. GILBERT; Ian M. KERR; Daniel H. WRESCHNER


Book ID
115120577
Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
744 KB
Volume
115
Category
Article
ISSN
1432-1327

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Nucleotides part XXX Chemical synthesis
✍ Ramamurthy Charubala; Wolfgang Pfleiderer; Robert W. Sobol; Shi Wu Li; Robert J. 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 German ⚖ 511 KB

The chemical synthesis of adenylyl-(2-5')-adenylyl-(2-5')-8-azidoadenosine (15) was performed by the phosphotriester approach. Enzymatic phosphorylation of 15 by [y -3ZP]ATP led to the corresponding labelled 5'monophosphate 16. Photoinsertion of 16 took place on UV irradiation by covalent cross link