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Nucleotides part XXX Chemical synthesis of adenylyl-(2′ → 5′)-adenylyl-(2′ → 5′)-8-azidoadenosine, and activation and photoaffinity labelling of RNase L by [32P]p5′A2′p5′A2′p5′N38A

✍ Scribed by Ramamurthy Charubala; Wolfgang Pfleiderer; Robert W. Sobol; Shi Wu Li; Robert J. Suhadolnik

Publisher
John Wiley and Sons
Year
1989
Tongue
German
Weight
511 KB
Volume
72
Category
Article
ISSN
0018-019X

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✦ Synopsis

The chemical synthesis of adenylyl-(2-5')-adenylyl-(2-5')-8-azidoadenosine (15) was performed by the phosphotriester approach. Enzymatic phosphorylation of 15 by [y -3ZP]ATP led to the corresponding labelled 5'monophosphate 16. Photoinsertion of 16 took place on UV irradiation by covalent cross linking to a protein of M, 80 K known to be RNase L. Radiobinding and core-cellulose assays as well as photoaffinity labelling experiments with 16 are described.

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