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Synthesis and use of peptide substrates for the isolation and characterization of proenkephalin processing enzymes

✍ Scribed by MARET, GERARD ;BLANOT, DIDIER ;WELTI, MANFRED ;FAUCHERE, JEAN-LUC

Book ID
115098639
Publisher
Wiley (Blackwell Publishing)
Year
2009
Tongue
English
Weight
358 KB
Volume
29
Category
Article
ISSN
0367-8377

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Enzymes hydrolyzing chymotrypsin synthetic substrate and trypsin synthetic substrate, referred to as Enzyme I and Enzyme II, respectively, were found in the envelope fraction of Capnocytophaga gingivalis (ATCC 33624). Detergent extraction of both enzymes were purified by gel filtration, ion exchang