Tritiated uridine-5'-diphosphogalactose (UDP-[3H]Gal) has been widely used to study oligosaccharide biosynthesis and structure. It can be synthesized either chemically or enzymatically using galactose oxidase to oxidize the hydroxyl moiety at C-6 to an aldehyde (6-aldo-UDP-Gal), which is then reduce
Synthesis and rapid purification of UDP-[6-3H]galactose
β Scribed by Barbara Fudem-Goldin; Pamela Voulalas; George A. Orr
- Book ID
- 118387844
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 232 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0165-022X
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Glycosyltransferases enzymatically transfer monosaccharides from sugar-nucleotides to complex oligosaccharide chains and, as cell surface molecules, exhibit receptor-like activity. We have modified the substate UDP-galactose to produce a compound that has useful absorbance and fluorescence propertie
Simple procedures for the preparation of UDP-D-[U-'\*C]galBCtUrOniC acid and UDP-D-[6+i]gatactose with high specific activities are described. The conversion involves the enzymatic oxidation of the appropriate UDP-o-galactose derivative to UDP-o-galactose-6aldehyde followed by either further oxidat
## Abstract Galactofuranose metabolism is a good target for the development of novel chemotherapeutic agents for the treatment of some microbial infections. This is a valid objective because galactofuranose is absent in mammals. Two enzymes are involved in the biosynthesis of molecules containing g