## Abstract Cytokines produced by immune system cells infiltrating pancreatic islets are candidate mediators of islet beta‐cell destruction in autoimmune insulin‐dependent diabetes mellitus. After 72 h exposure of human pancreatic islets to a cytotoxic cytokine combination of interleukin 1 beta (50
Synthesis and Properties of a Mitochondrial Peripheral Benzodiazepine Receptor Conjugate
✍ Scribed by Abdessamad El Alaoui; Frédéric Schmidt; Marianne Sarr; Didier Decaudin; Jean-Claude Florent; Ludger Johannes
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 393 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1860-7179
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Benzodiazepine (BDZ) binding sites were studied by using 3H-diazepam and 3H-Ro 5-4864 in intact lymphocytes from peripheral blood (PBL), in comparison to kidney and cerebellum. Experiments with 3H-diazepam performed at equilibrium and measuring kinetics revealed that BDZ binding sites are indeed pre
## Abstract The synthesis of the [^3^H] labelled peripheral benzodiazepine receptor (PBR) ligand CB 34, useful for binding assay, tissue distribution studies, and elucidation of the physiological role of PBR, is described. Catalytic reduction with ^3^H~2~ gas and 10% Pd/C catalyst of the key interm
A nonisotopic receptor assay using the biotin-1012-S conjugate was developed and the usefulness of this conjugate as a probe ligand for the benzodiazepine receptor was evaluated. The conjugate was incubated in a receptor suspension, and then the concentration of free conjugate in the supernatant was