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Synthesis and properties of 5-membered heterocyclic disulfides: Application for enzyme modification

โœ Scribed by U. Schmidt; G. Pfleiderer; F. Bartkowiak

Book ID
102629400
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
500 KB
Volume
138
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

Synthesis of the disulfides 2,2'dithiodithiazole, 2,2'dithiobis(4-methylthiazole), and S,S-dithiobis(3-methylisothiazole), which are thiol-specific reagents, is described and the uv-absorption spectra of the disulfides and corresponding thiols and/or thiones are presented. The utility of these and several other reagents for the modification of thiol groups in enzymes has been demonstrated with lactate dehydrogenax and malate dehydrogenase and compared with the frequently applied modification reagents 5,5'dithiobis(2nitrobenzoic acid) and 2,2'-dithiodipyridine. The 5-membered heterocyclic disulfides permit the spectrophotometric determination of all sulthydryl groups of malate dehydrogenase and lactate dehydrogenase without requiring their prior denaturation. Application of these reagents makes possible the determination of SH groups in the pH range 67.

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