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Synthesis and processing of Escherichia coli TEM-β-lactamase and Bacillus licheniformis α-amylase in E. coli: The role of signal peptidase I

✍ Scribed by Maarten van Dijl, Jan ;Smith, Hilde ;Bron, Sierd ;Venema, Gerard

Publisher
Springer
Year
1988
Tongue
English
Weight
826 KB
Volume
214
Category
Article
ISSN
0026-8925

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✦ Synopsis

A mutant of Escherichia coli, in which signal peptidase I synthesis can be regulated, was constructed. The mutant was used to study the effects of signal peptidase I limitation on the synthesis and efficiency of processing of two proteins: the periplasmic E. coli TEM-beta-lactamase and Bacillus licheniformis alpha-amylase, which also accumulates in the periplasm of E. coli. Signal peptidase I limitation resulted in reduced rates of processing of pre-beta-lactamase and in strong inhibition of synthesis of alpha-amylase. The data suggest that beta-lactamase is processed post-translationally and that an intimate relationship exists between the synthesis and processing of alpha-amylase.

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