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Molecular cloning of the gene for the β-lactamase of Bacillus licheniformis and its expression in Escherichia coli

✍ Scribed by Brammar, William J. ;Muir, Shirley ;McMorris, Anne

Publisher
Springer
Year
1980
Tongue
English
Weight
932 KB
Volume
178
Category
Article
ISSN
0026-8925

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✦ Synopsis

The structural gene, pen, for the beta-lactamase of B. licheniformis has been cloned into a lambda vector and shown to be expressed at a low rate in E. coli. The cloned pen gene appears to be expressed from a promoter within the fragment of B. licheniformia DNA, since its rate of expression is not affected by the presence of the phage repressor, the absence of the phage's positive-control functions, or the position or orientation of the gene within the phage genome.

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