Synthesis and Luminescence Spectral Characterization of Long-Lifetime Lipid Metal–Ligand Probes

✍ Scribed by Li Li; Henryk Szmacinski; Joseph R. Lakowicz

• Publisher: Elsevier Science
• Year: 1997
• Tongue: English
• Weight: 142 KB
• Volume: 244
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1996.9869

✦ Synopsis

change in anisotropy at the phase transition tempera-We synthesized phospholipid analogues of phosphature of membranes ( ). The extension of the anisotropy tidyl ethanolamine which contains a ruthenium measurements to include time-resolved anisotropies metal-ligand complex (MLC) covalently bound to the resulted in increased understanding of the order and amino group. Two analogues were synthesized, condynamics of cell and model membranes (8-13).

The first lipid probe contains the mono-carboxy derivaa-phosphatidylglycerol; MLC, metal -ligand complex; Ru -PE, the tive Ru(bpy) 2 (mcbpy) which is linked to a single moleconjugate of Ru(bpy) 2 (mcbpy) with one PE molecule; Ru -PE 2 , the cule of phosphatidylethanolamine (PE) (Scheme I). The conjugate of Ru(bpy) 2 (dcbpy) with two PE molecules; FD, frespectral properties of the initial probe were described quency domain; DPH, 1,6-diphenyl-1,3,5-hexatriene; NHS, N-hydroxysuccinimide; DMF, dimethylformamide.

briefly in a preliminary communication (20). In the sec-80