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Long-lifetime lipid probe containing a luminescent metal-ligand complex

✍ Scribed by Li Li; Henryk Szmacinski; Joseph R. Lakowicz

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 135 KB
Volume: 3
Category: Article
ISSN: 1075-4261
DOI: 10.1002/(sici)1520-6343(1997)3:2<155::aid-bspy8>3.0.co;2-6

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✦ Synopsis

We describe the chemical synthesis and spectral properties of a long-lifetime luminescent probe for membranes. A ruthenium metal-ligand complex was covalently coupled to the amino group of phosphatidyl ethanolamine. When incorporated into model membranes, this probe displays decay times near 500 ns. Importantly, the probe displays polarized emission and can be used to study membrane motions on the microsecond timescale.

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